inflammatory protein biomarkers  (Thermo Fisher)

Journal: World Journal of Diabetes

Article Title: What can we learn from β-cell failure biomarker application in diabetes in childhood? A systematic review

doi: 10.4239/wjd.v12.i8.1325

Figure Lengend Snippet: Overview of the included studies with their corresponding quality assessment

Article Snippet: Babar et al [ ], 2011 , United States , Vascular inflammatory biomarkers (hs-CRP, Homocysteine C-reactive protein) , hs-CRP, Homocysteine C-reactive protein , hs-CRP → solid- phase ELISA from MP Biomedicals (West Chester, PA); Homocysteine→ Siemens Immulite platforms and kit (Diagnostic Products, Flanders, NJ) , No pretreatment was specified , Solid-phase ELISAs; Chemilumine scence , Sample size: 36 children, 21 with T1DM (aged 8.3 ± 0.3 yr with a diabetes duration of 4.3 ± 0.4 yr) and 15 group-matched healthy siblings (aged 7.6 ± 0.3 yr) , Known dyslipidemia, hypertension, microvascular complications, anemia (hemoglobin, 11.0 g/dL), congenital heart disease, allergy to ultrasound gel, or family history of hypercholesterolemia or premature cardiovascular disease , Yes , Age, sex, BMI, blood pressure, blood cell count, plasma glucose, HbA1c, lipids, hs-CRP, fibrinogen, chemistry panel, homocysteine, and erythrocyte folate, lipid profile. Diabetes duration in study group. Insulin regimen and dose , T1DM preadolescent children showed increased vascular inflammation and presented with higher fasting plasma glucose, HbA1c and hs-CRP; the flow-mediated dilatation was attenuated; this suggests endothelial dysfunction, harbingers of cardiovascular risk , Vascular inflammatory biomarkers. Endothelial dysfunction marker; Fasting plasma glucose, oral glucose tolerance curve, lipid profile, HbA1c, hs-CRP, fibrinogen, homocysteine, and erythrocyte (red blood cell) , Macroangiopathy.

Techniques: Biomarker Assay, Permeability, Molecular Weight, Infection, Expressing, Transgenic Assay, Functional Assay, In Situ, In Vivo

Journal: World Journal of Diabetes

Article Title: What can we learn from β-cell failure biomarker application in diabetes in childhood? A systematic review

doi: 10.4239/wjd.v12.i8.1325

Figure Lengend Snippet: Overview of the articles included with the study characteristics

Article Snippet: Babar et al [ ], 2011 , United States , Vascular inflammatory biomarkers (hs-CRP, Homocysteine C-reactive protein) , hs-CRP, Homocysteine C-reactive protein , hs-CRP → solid- phase ELISA from MP Biomedicals (West Chester, PA); Homocysteine→ Siemens Immulite platforms and kit (Diagnostic Products, Flanders, NJ) , No pretreatment was specified , Solid-phase ELISAs; Chemilumine scence , Sample size: 36 children, 21 with T1DM (aged 8.3 ± 0.3 yr with a diabetes duration of 4.3 ± 0.4 yr) and 15 group-matched healthy siblings (aged 7.6 ± 0.3 yr) , Known dyslipidemia, hypertension, microvascular complications, anemia (hemoglobin, 11.0 g/dL), congenital heart disease, allergy to ultrasound gel, or family history of hypercholesterolemia or premature cardiovascular disease , Yes , Age, sex, BMI, blood pressure, blood cell count, plasma glucose, HbA1c, lipids, hs-CRP, fibrinogen, chemistry panel, homocysteine, and erythrocyte folate, lipid profile. Diabetes duration in study group. Insulin regimen and dose , T1DM preadolescent children showed increased vascular inflammation and presented with higher fasting plasma glucose, HbA1c and hs-CRP; the flow-mediated dilatation was attenuated; this suggests endothelial dysfunction, harbingers of cardiovascular risk , Vascular inflammatory biomarkers. Endothelial dysfunction marker; Fasting plasma glucose, oral glucose tolerance curve, lipid profile, HbA1c, hs-CRP, fibrinogen, homocysteine, and erythrocyte (red blood cell) , Macroangiopathy.

Techniques: Biomarker Assay, Multiple Displacement Amplification, Colorimetric Assay, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Cell Counting, Marker, Incubation, Lysis, Flow Cytometry, Gas Chromatography-Mass Spectrometry, Activity Assay, Centrifugation, Infection, Isolation, Real-time Polymerase Chain Reaction, Derivative Assay, Immunohistochemistry, Functional Assay, Expressing, Next-Generation Sequencing, Sequencing, Software, Cytometry, Luminex, Staining, Blocking Assay, In Situ, Fluorescence, In Vivo, Immunofluorescence, Concentration Assay, Agglutination, Sandwich ELISA, Cell Function Assay, Protein Extraction, Bicinchoninic Acid Protein Assay, Western Blot

Journal: World Journal of Diabetes

Article Title: What can we learn from β-cell failure biomarker application in diabetes in childhood? A systematic review

doi: 10.4239/wjd.v12.i8.1325

Figure Lengend Snippet: Overview of the articles included that studied insulin resistance and Beta cell mass

Article Snippet: Babar et al [ ], 2011 , United States , Vascular inflammatory biomarkers (hs-CRP, Homocysteine C-reactive protein) , hs-CRP, Homocysteine C-reactive protein , hs-CRP → solid- phase ELISA from MP Biomedicals (West Chester, PA); Homocysteine→ Siemens Immulite platforms and kit (Diagnostic Products, Flanders, NJ) , No pretreatment was specified , Solid-phase ELISAs; Chemilumine scence , Sample size: 36 children, 21 with T1DM (aged 8.3 ± 0.3 yr with a diabetes duration of 4.3 ± 0.4 yr) and 15 group-matched healthy siblings (aged 7.6 ± 0.3 yr) , Known dyslipidemia, hypertension, microvascular complications, anemia (hemoglobin, 11.0 g/dL), congenital heart disease, allergy to ultrasound gel, or family history of hypercholesterolemia or premature cardiovascular disease , Yes , Age, sex, BMI, blood pressure, blood cell count, plasma glucose, HbA1c, lipids, hs-CRP, fibrinogen, chemistry panel, homocysteine, and erythrocyte folate, lipid profile. Diabetes duration in study group. Insulin regimen and dose , T1DM preadolescent children showed increased vascular inflammation and presented with higher fasting plasma glucose, HbA1c and hs-CRP; the flow-mediated dilatation was attenuated; this suggests endothelial dysfunction, harbingers of cardiovascular risk , Vascular inflammatory biomarkers. Endothelial dysfunction marker; Fasting plasma glucose, oral glucose tolerance curve, lipid profile, HbA1c, hs-CRP, fibrinogen, homocysteine, and erythrocyte (red blood cell) , Macroangiopathy.

Techniques: Activity Assay, Infection, Expressing, Functional Assay, Positron Emission Tomography, Injection, Immunohistochemistry, Histopathology, Light Microscopy, H&E Stain

Journal: World Journal of Diabetes

Article Title: What can we learn from β-cell failure biomarker application in diabetes in childhood? A systematic review

doi: 10.4239/wjd.v12.i8.1325

Figure Lengend Snippet: Global view of the main biomarkers analyzed on the articles included for this review. The biomarkers are divided into the ones that substantially appear on early beta cell damage (characterized by increased insulin and lipid and islet amyloid polypeptide secretion) and increased insulin resistance and of late beta cell damage (characterized by increased oxidative stress and insulin resistance, a markedly insulin secretion and an impaired mitochondrial function, which translates into islet amyloid polypeptide fibrillation). RIAO: Real human islet amyloid polypeptide amyloid oligomers; FMD: Flow-mediated dilatation; CAM: Cytoadhesive molecule; hs-CRP: High-sensitivity C-reactive protein.

Article Snippet: Babar et al [ ], 2011 , United States , Vascular inflammatory biomarkers (hs-CRP, Homocysteine C-reactive protein) , hs-CRP, Homocysteine C-reactive protein , hs-CRP → solid- phase ELISA from MP Biomedicals (West Chester, PA); Homocysteine→ Siemens Immulite platforms and kit (Diagnostic Products, Flanders, NJ) , No pretreatment was specified , Solid-phase ELISAs; Chemilumine scence , Sample size: 36 children, 21 with T1DM (aged 8.3 ± 0.3 yr with a diabetes duration of 4.3 ± 0.4 yr) and 15 group-matched healthy siblings (aged 7.6 ± 0.3 yr) , Known dyslipidemia, hypertension, microvascular complications, anemia (hemoglobin, 11.0 g/dL), congenital heart disease, allergy to ultrasound gel, or family history of hypercholesterolemia or premature cardiovascular disease , Yes , Age, sex, BMI, blood pressure, blood cell count, plasma glucose, HbA1c, lipids, hs-CRP, fibrinogen, chemistry panel, homocysteine, and erythrocyte folate, lipid profile. Diabetes duration in study group. Insulin regimen and dose , T1DM preadolescent children showed increased vascular inflammation and presented with higher fasting plasma glucose, HbA1c and hs-CRP; the flow-mediated dilatation was attenuated; this suggests endothelial dysfunction, harbingers of cardiovascular risk , Vascular inflammatory biomarkers. Endothelial dysfunction marker; Fasting plasma glucose, oral glucose tolerance curve, lipid profile, HbA1c, hs-CRP, fibrinogen, homocysteine, and erythrocyte (red blood cell) , Macroangiopathy.

Techniques:

Journal: Frontiers in Physiology

Article Title: Suppressed Vascular Leakage and Myocardial Edema Improve Outcome From Myocardial Infarction

doi: 10.3389/fphys.2020.00763

Figure Lengend Snippet: Inflammation, neutrophil infiltration and MPO levels in conjunction with MI. (A) Immunostaining for CD45 (green) and CD31 (red) to visualize infiltrating leukocytes and the vasculature, respectively, in the infarcted areas of WT and Vegfr2 Y 949 F / Y 949 F hearts at 3 days post-MI. DAPI (blue) shows nuclei. Scale bar, 100 μm. (B,C) Quantification of CD45-positive and CD31-positive area/field ( n = 4–5 individual hearts/strain, 5 fields/section, and 3 sections/heart) in the infarcted part of the heart shows no significant differences using Mann–Whitney test. (D) Myeloperoxidase (MPO) protein levels in heart lysate measured using a multiplex inflammatory protein biomarker panel ( n = 10 individual heart lysates/strain). Mann–Whitney test * p < 0.05. (E) Immunostaining for Ly6G (yellow) and CD45 (magenta) in the infarcted area of WT and Vegfr2 Y 949 F / Y 949 F hearts at 3 days after MI. Hoechst (blue) shows nuclei. Scale bar, 100 μm. (F) Quantification of Ly6G area normalized to CD31 area in the infarcted part of the heart ( n = 6–7 individual hearts/strain, 5 fields/section, and 3 sections/heart) shows no significant differences using Mann–Whitney test. For all panels, n refers to biological replicates.

Article Snippet: Heart lysate from the lower half of the heart (about 5 mm) were analyzed using a multiplex human inflammatory protein biomarker panel (Olink Bioscience; see www.olink.com/content/uploads/2015/12/0696-v1.3-Proseek-Multiplex-CVD-I-Validation-Data_final.pdf ). where reactivity depended on detected by two antibodies against each marker.

Techniques: Immunostaining, MANN-WHITNEY, Multiplex Assay, Biomarker Discovery